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If your laser output is mirrored horizontally or vertically, move the dot to the opposite corner, horizontally or vertically, depending on the direction that the output is mirrored, and that will correct it. If you have a DSP laser, like Ruida or Trocen, the origin is usually where the limit switches are placed, and will will be the corner the laser seeks when powered up. If you have a GCode based system, this is almost always at the front left, regardless of the location of your limit switches. This is the origin corner or 0,0 location for your laser.
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Note that this setting on GRBL & DSP devices does not affect the laser itself, it's just to tell LightBurn how large the work area of the laser is. Set this to the maximum X and Y travel for your laser. This is the working size of your laser bed.
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I encourage you to get a copy of the Rambo firmware, and do a diff between the configuration.h and configuration_adv.h of the Rambo firmware and your firmware.
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Sometimes it defines a PWM value in the range of 0 to 255, sometimes it is spindle speed. The ‘S’ parameter can be interpreted in different ways depending on your settings. With that assumption my best guess is that you have an issue with how you have set up the firmware. You are using a 12864 LCD Display, and you can both turn the laser on and control the strength using the display?
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